B M B 445W
Laboratory in Molecular Genetics I (2) Laboratory in molecular techniques in gene analysis and microbial genetics, emphasizing in vitro methodologies.
B M B 445W Laboratory in Molecular Genetics I (2)
The objectives of B M B 445W are to provide advanced Biochemistry and Microbiology students with instruction in (1) techniques commonly used in modern research and clinical laboratories in this field, (2) evaluation of the quality of experimental data, including appropriate analysis, and (3) presentation of results of laboratory work in written form. Experiments are focused on key techniques and procedures such as DNA isolation, polymerase chain reaction, Southern hybridization analysis, and DNA sequencing. Students are evaluated via (1) written lab reports organized in the format found in most primary research journals in the field and (2) written examinations that assess the understanding of principles and methodology. B M B 445W is an extension of the nucleic acid section of B M B 342, which is a prerequisite for B M B 445W. The in vitro techniques presented in B M B 445W complement the in vivo techniques in B M B 446, though neither course is prerequisite for the other. B M B 445W is a requirement for the B M B major, and is an elective for other students, most notably Microbiology majors and graduate students in other areas of the life sciences. B M B 445W is taught in a standard biochemistry teaching laboratory facility that houses the required equipment necessary for analysis of DNA (electrophoresis units, centrifuges, thermocyclers, cold room, spectrophotometers).
Note : Class size, frequency of offering, and evaluation methods will vary by location and instructor. For these details check the specific course syllabus.